Experiments on Tracer Diffusion in Aqueous and Non-Aqueous Solvent Combinations

Forced Rayleigh scattering is used to study the tracer diffusion of an azobenzene in binary combinations of polar solvents, including water. In the absence of water, the tracer diffusion coefficient D in the mixture lies between the diffusion coefficients within the pure solvents, on a curve that is reasonably close to the prediction of free-volume theory. If water is present, on the other hand, the diffusion coefficient displays a minimum that is less than the smaller of the two pure-solvent values. We attempt to understand the different behavior in water by concentrating on the fairly hydrophobic nature of the solute, leading to a first solvent shell that is hydrophobic on the inside and hydrophilic on the outside. We also believe that clusters of amphiphiles explain the observation that, in aqueous combinations, D is nearly constant above a certain amphiphile mole fraction

Effective Exploration Behavior for Chemical-Sensing Robots

Mobile robots that can effectively detect chemical effluents could be useful in a variety of situations, such as disaster relief or drug sniffing. Such a robot might mimic biological systems that exhibit chemotaxis, which is movement towards or away from a chemical stimulant in the environment. Some existing robotic exploration algorithms that mimic chemotaxis suffer from the problems of getting stuck in local maxima and becoming “lost”, or unable to find the chemical if there is no initial detection. We introduce the use of the RapidCell algorithm for mobile robots exploring regions with potentially detectable chemical concentrations. The RapidCell algorithm mimics the biology behind the biased random walk of Escherichia coli (E. coli) bacteria more closely than traditional chemotaxis algorithms by simulating the chemical signaling pathways interior to the cell. For comparison, we implemented a classical chemotaxis controller and a controller based on RapidCell, then tested them in a variety of simulated and real environments (using phototaxis as a surrogate for chemotaxis). We also added simple obstacle avoidance behavior to explore how it affects the success of the algorithms. Both simulations and experiments showed that the RapidCell controller more fully explored the entire region of detectable chemical when compared with the classical controller. If there is no detectable chemical present, the RapidCell controller performs random walk in a much wider range, hence increasing the chance of encountering the chemical. We also simulated an environment with triple effluent to show that the RapidCell controller avoided being captured by the first encountered peak, which is a common issue for the classical controller. Our study demonstrates that mimicking the adapting sensory system of E. coli chemotaxis can help mobile robots to efficiently explore the environment while retaining their sensitivity to the chemical gradient

Cognitive Performance and Heart Rate Variability: The Influence of Fitness Level

In the present study, we investigated the relation between cognitive performance and heart rate variability as a function of fitness level. We measured the effect of three cognitive tasks (the psychomotor vigilance task, a temporal orienting task, and a duration discrimination task) on the heart rate variability of two groups of participants: a high-fit group and a low-fit group. Two major novel findings emerged from this study. First, the lowest values of heart rate variability were found during performance of the duration discrimination task, compared to the other two tasks. Second, the results showed a decrement in heart rate variability as a function of the time on task, although only in the low-fit group. Moreover, the high-fit group showed overall faster reaction times than the low-fit group in the psychomotor vigilance task, while there were not significant differences in performance between the two groups of participants in the other two cognitive tasks. In sum, our results highlighted the influence of cognitive processing on heart rate variability. Importantly, both behavioral and physiological results suggested that the main benefit obtained as a result of fitness level appeared to be associated with processes involving sustained attention.This research was supported by the Spanish Ministerio de Educación y Cultura with a predoctoral grant (FPU-AP2010-3630) to the first author, Spanish grants SEJ2007-63645 from the Junta de Andalucía to Daniel Sanabria, Mikel Zabala and Esther Morales, and the CSD2008-00048 CONSOLIDER INGENIO (Dirección General de Investigación) to Daniel Sanabria

The Impact of Intermittent Umbilical Cord Occlusions on the Inflammatory Response in Pre-Term Fetal Sheep

Fetal hypoxic episodes may occur antepartum with the potential to induce systemic and cerebral inflammatory responses thereby contributing to brain injury. We hypothesized that intermittent umbilical cord occlusions (UCOs) of sufficient severity but without cumulative acidosis will lead to a fetal inflammatory response. Thirty-one chronically instrumented fetal sheep at ∼0.85 of gestation underwent four consecutive days of hourly UCOs from one to three minutes duration for six hours each day. Maternal and fetal blood samples were taken for blood gases/pH and plasma interleukin (IL)-1β and IL-6 levels. Animals were euthanized at the end of experimental study with brain tissue processed for subsequent counting of microglia and mast cells. Intermittent UCOs resulted in transitory fetal hypoxemia with associated acidemia which progressively worsened the longer umbilical blood flow was occluded, but with no cumulative blood gas or pH changes over the four days of study. Fetal arterial IL-1β and IL-6 values showed no significant change regardless of the severity of the UCOs, nor was there any evident impact on the microglia and mast cell counts for any of the brain regions studied. Accordingly, intermittent UCOs of up to three minutes duration with severe, but limited fetal hypoxemia and no cumulative acidemia, do not result in either a systemic or brain inflammatory response in the pre-term ovine fetus. However, fetal IL-1B and IL-6 values were found to be well correlated with corresponding maternal values supporting the placenta as a primary source for these cytokines with related secretion into both circulations. Female fetuses were also found to have higher IL-1β levels than males, indicating that gender may impact on the fetal inflammatory response to various stimuli

On the roles of Notch, Delta, kuzbanian, and inscuteable during the development of Drosophila embryonic neuroblast lineages

10.1016/j.ydbio.2009.09.030Developmental Biology3362156-168DEBI